Nucleosomes restrict the occupancy of most transcription factors (TF) by reducing binding and accelerating dissociation, while a small group of TFs have high affinities to nucleosome-embedded sites and facilitate nucleosome displacement.To understand this process mechanistically, we investigated two Saccharomyces cerevisiae TFs, Reb1 and Cbf1.We sh
Protein Aggregation Mediated by Cysteine Oxidation During the Stacking Phase of Discontinuous Buffer SDS-PAGE
The resolution of complex protein mixtures by discontinuous buffer SDS-PAGE is Reclaimers accomplished by their concentration into thin bands in the stacking gel, followed by their separation during migration through the resolving gel.Recombinant human interferon-inducible protein-10 (IP-10), a 10-kDa C-X-C chemokine with four cysteines, aggregated
A comparison between allogeneic stem cell transplantation from unmanipulated haploidentical and unrelated donors in acute leukemia
Abstract Background In the absence of a HLA-matched related or matched unrelated donor, allogeneic stem cell transplantation (allo-SCT) from mismatched unrelated donors or haploidentical donors are potential alternatives for patients with acute leukemia with an indication to allo-SCT.The objective of this study was to compare the outcome of allo-SC
The effect of whole blood logistics on neutrophil non-specific activation and kinetics ex vivo
Abstract While the exquisite sensitivity of neutrophils enables their rapid response to infection in vivo; this same sensitivity complicates the ex vivo study of neutrophils.Handling of neutrophils ex vivo is fraught with unwanted heterogeneity and alterations that can diminish the reproducibility of assays and limit what biological conclusions can
Where there is pancreatic juice, there is a way: Spontaneous fistulization of severe acute pancreatitis-associated collection into urinary bladder
Pancreatic fluid collections (PFC) are notorious for their extension beyond Openers the normal confines of the pancreatic bed.This distribution is explained by dissection along the fascial planes in retroperitoneum due to the digestive enzymes within the PFC.In genitourinary track, PFCs have been described to involve the kidneys and the ureters.We